Part:BBa_K1746002:Design

TdT GIP 213-215 subAAA (Mutated TdT Variant)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 154
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1384
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 933
  

Design Notes

This DNA sequence was based on a truncated variant of bovine TdT which was then codon optimized for optimal expression in E. Coli, and chemically synthesized.

Source

This DNA sequence was chemically synthesized and codon optimized for expression in E. Coli.